Loading...
This record has been deleted. Since it is linked to a published record, it is available here for reference purposes only.
Click here to view the deleted recordThis document has been updated. This is not the latest published version. Click here to view the latest version of the record.
Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Antisense hybrid aspen modified for altered circadian rhythms
EN
asPHYA
No
-
Person:Dr Stefan JanssonProfessor in plant cell and mollecular biology, Umeå Plant Science Centre, Physioogical BotanyUmeå University Umeå Plant Science Centre Physiological BotanyUmeå, Umeå
901 87, SwedenPhone: +4690-7865354,Fax:Email: stefan.jansson@umu.se,Website:Related OrganizationSwedish Agricultural University, Umeå (SLU)Academic or research instituteUmeå University Umeå Plant Science Centre Physiological BotanyUmeå, Umeå
901 87, SwedenPhone: +4690-7865354,Fax:Email: stefan.jansson@umu.se,Website:
The modified aspens grow similarly to wild-type hybrid aspens, except demonstrating an altered seasonally-regulated growth. The antisense expression of a Phytochrome A (PttPHYA) transcript, which elicits gene silencing of the endogenous PttPHYA, causes the modified trees to show an earlier growth cessation, earlier bud formation in response to shorter daylengths, reduced red-light sensitivity, longer period of leaf motion, invertion at a longer day than wild-type trees, an altered expression of clock related genes, and a longer period of clock-controlled gene expression.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
-
BCH-ORGA-SCBD-105731-2 Organism Populus tremula x Populus tremuloides (Hybrid aspen)Trees
Populus tremula x Populus tremuloides WT clone T89
EN
pPCV702
EN
- Agrobacterium-mediated DNA transfer
|
0.290 kb
|
|
0.800 kb
|
|
0.410 kb
|
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
-
BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
-
BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
-
BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene Promoter | Agrobacterium tumefaciens (Agrobacterium)Promoter
-
BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Kanamycin)
-
BCH-GENE-SCBD-104314-4 Gene 4 transcription terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
-
BCH-GENE-SCBD-114963-1 Beta-lactamase promoter | Escherichia coli (ECOLX)Promoter
-
BCH-GENE-SCBD-14975-5 Beta-lactamase gene | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Ampicillin)
-
BCH-GENE-SCBD-114964-1 Beta-lactamase terminator | Escherichia coli (ECOLX)Terminator
-
BCH-GENE-SCBD-114966-2 Phytochome A | Populus tremula x Populus tremuloides (Hybrid aspen)Protein coding sequence | Changes in physiology and/or production (Growth rate, Photoperiod response)
The transformation of the aspen trees introduced three gene cassettes: an antisense (Populus tremula x Populus tremuloides) PHYTOCHROMEA (PttPHYA) cassette, an Escherichia coli neomycin phosphotransferase II (NptII) cassette, and an E. coli beta-lactamase (AmpR) cassette.
Transcription of the antisense PttPHYA is driven by the Cauliflower Mosaic Virus 35S promoter and terminates at the Agrobacterium tumefaciens nopaline synthase (nos) gene terminator. PttPHYA is the full-length sequence synthesized as cDNA from the mRNA and therefore no introns are present. PttPHYA was inserted in reverse to create an RNA with a complementary sequence to the endogenous messenger RNA.
Transcription of NptII is under the control of the A. tumefaciens nos promoter and the A. tumefaciens gene 4 terminator. NptII is used as a selectable marker for plant transformation events as it confers resistance to kanamycin.
AmpR expression is under the control of the native beta-lactamase promoter and terminator. Expression is restrained to bacterial systems and transcription is not expected to occur in eukaryotic systems. This allows for selection using beta-lactam containing antibiotics during bacterial transformation. The cassette was taken from the pBR322 plasmid.
EN
Transcription of the antisense PttPHYA is driven by the Cauliflower Mosaic Virus 35S promoter and terminates at the Agrobacterium tumefaciens nopaline synthase (nos) gene terminator. PttPHYA is the full-length sequence synthesized as cDNA from the mRNA and therefore no introns are present. PttPHYA was inserted in reverse to create an RNA with a complementary sequence to the endogenous messenger RNA.
Transcription of NptII is under the control of the A. tumefaciens nos promoter and the A. tumefaciens gene 4 terminator. NptII is used as a selectable marker for plant transformation events as it confers resistance to kanamycin.
AmpR expression is under the control of the native beta-lactamase promoter and terminator. Expression is restrained to bacterial systems and transcription is not expected to occur in eukaryotic systems. This allows for selection using beta-lactam containing antibiotics during bacterial transformation. The cassette was taken from the pBR322 plasmid.
-
BCH-GENE-SCBD-114966-2 Phytochome A | Populus tremula x Populus tremuloides (Hybrid aspen)Protein coding sequence | Changes in physiology and/or production (Growth rate, Photoperiod response)
Due to the reverse orientation of the PttPHYA sequence in the vector, RNA molecules are produced with complementarity to the endogenous PttPHYA transcript. The complementary RNA molecules will base pair to the endogenous transcript and form double stranded RNA molecules, which can elicit an RNA interference response and cause targeted degradation of mRNA molecules with similar sequence. Thus, silencing of the endogenous PttPHYA expression will occur.
EN
- Research
EN
EN
